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Hung King Tiong, Ph.D.

Assistant Professor of Biology (Microbiology)

Employment Date: Fall 2017

Classes taught:

  • BY101 General Biology
  • BY104 - Principles of Biology
  • BY340 – General Microbiology
  • BY340L – Microbiology Laboratory 
  • BY389 – Lab Practicum
  • BY408/598 – Food Microbiology
  • BY495 – Research in Biology
  • BY597 – Microbial Survey
  • BY599 – Thesis in Biological Sciences

Committee:

  • Health Professions Advising, UWA. Aug 2017-present

  • University Scholar, UWA. Aug 2017-present

  • University Research Symposium, UWA. Oct 2017-present

Research area: Dr. Tiong is involved in molecular research of food-associated microorganisms in the areas of detection, isolation, identification (16S rRNA gene sequencing), virulence characterization of foodborne pathogens and their toxins (adherent Listeria, Vibrio spp., shiga toxins), the use of antimicrobials to inhibit foodborne pathogens and spoilage microorganisms (bacteriocins, thermal destruction, intervention chemicals), and verification of food safety processes using inoculated validation studies.

Professional biography:

  • Assistant Professor, Department of Biological and Environmental Sciences, University of West Alabama (2017-present)

  • Post-doc Research Associate, Department of Animal Sciences, Oklahoma State University (2016-2017)

  • Ph.D. Graduate Research Associate, Department of Animal Science, Oklahoma State University (2009-2016)

  • Ph.D. Graduate Teaching Associate, Department of Animal Science, Oklahoma State University (2013-2017)

Selected presentations at professional meetings:

  • Isolation and Characterization of Vibrio parahaemolyticus Protected from Laboratory Validation from Natural Seafood Sources. International Association for Food Protection (IAFP), July 22, 2019.

  • Isolation of Antimicrobial- and Lactase-Producing Lactic Acid Bacteria from Farm Animals and Produce. IAFP, July 23, 2019.

  • Postharvest Interventions to Improve Quality and Microbial Safety of Microgreens. Institute of Food Technologists. Institute of Food Technologists (IFT), June 5, 2019.

  • Culture-based Isolation and Identification of Soil Microorganisms for Potential Soil Regenerating and Agricultural Benefits. UWA University Research Symposium (URS), March 5, 2019.

  • Regenerating Agricultural Soil of the Black Belt Region Using Bio-fertilizer. URS, March 6, 2018.

  • Identification of Putative Surface Proteins Involved in Adherence of L. monocytogenes on Abiotic Surfaces. IAFP, July 19, 2017.

  • Analysis of Surface Proteomics involved in Adherence of L. monocytogenes to Abiotic Surfaces. OSU Food & Agricultural Products Center/Institute of Food Technologists-Oklahoma (FAPC/IFT) Research Symposium, Feb 2017.

  • DNA Array of Select Genes of Two Surface-Adherent Phenotypes of L. monocytogenes Using PCR for Identification of Surface Adhesins. OSU FAPC/IFT-Oklahoma Research Symposium, Feb 2016.

  • Orbitrap Mass Spectrometry and qRT-PCR Analysis of Gene Expression to Identify the Molecular Basis of Bacterial Adherence in L. monocytogenes. OSU FAPC/IFT-Oklahoma Research Symposium, Feb 2013.

  • Genetics of Hydrogen Peroxide Production in S. pneumonia. American Society for Microbiology (ASM of Missouri branch), 2008.

Recent grants:

  • UWA Faculty Seed Grant (2019-2020; 2017-2018)

  • UWA Student Seed Grant (2019-2020; 2017-2018)

  • USDA Specialty Crop BLOCK Grant (2018-2019)

  • UWA Internal Research Grant (2017-present)

Education

  • Ph.D. - Oklahoma State University, Stillwater, OK Food Science (Microbiology) 2016
  • M.S. - Oklahoma State University, Stillwater, OK Microbiology & Molecular Genetics
  • B.S. - Oklahoma State University, Stillwater, OK Plant & Soil Sciences (Biotechnology)
  • Recent Publications

  • Tiong, H.K. and P.M. Muriana Identification of Putative Surface Proteins Involved in Adherence of Listeria monocytogenes on Abiotic Surfaces. https://iafp.confex.com/iafp/2017/webprogram/Paper15617.html 2017
  • Tiong, H.K. and P.M. Muriana. RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes. Pathogens 5(4):60. doi:10.3390/pathogens5040060 2016
  • Tiong, H.K., S. Hartson, and P.M. Muriana. Comparison of surface proteomes of adherence variants of Listeria monocytogenes using LC-MS/MS for identification of surface adhesins. Pathogens. 2016 Jun; 5(2):40. doi:10.3390/pathogens5020040 2016
  • Tiong, H.K., S. Hartson, and P.M. Muriana. Comparison of five methods for direct extraction of surface proteins from Listeria monocytogenes for proteomic analysis by Orbitrap mass spectrometry. J. Microbiol. Methods 110: 54-60. doi:10.1016/j.mimet.2015.01.004 2015