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Hung King Tiong, Ph.D.

Assistant Professor of Biology (Microbiology)

Employment Date: Fall 2017

Classes taught:

  • BY101 Introductory Biology I
  • BY103  Honors Biology
  • BY104 Principles of Biology
  • BY307 Special Topic in Biology
  • BY308 Seminar in Biology
  • BY340 – General Microbiology
  • BY340L – Microbiology Laboratory 
  • BY389 – Lab Practicum
  • BY443/543 (408/598) – Food Microbiology
  • BY495 – Research in Biology
  • BY597 – Microbial Survey
  • BY599 – Thesis in Biological Sciences

Online courses taught:

  • BY340-61 General Microbiology (lab included) Online (SP/SU/FA 1 and 2)
  • BY443-61/543-91 (408-61/598-91)  Food Microbiology (lab included) Online (SP/SU/FA 1 and 2)

Committee:

  • Health Professions Advising, UWA. Aug 2017-present
  • University Scholar, UWA. Aug 2017-present
  • University Research Symposium, UWA. Oct 2017-present

Research area: Dr. Tiong is involved in molecular research of food-associated microorganisms in the areas of detection, isolation, identification (16S rRNA gene sequencing), virulence characterization of foodborne pathogens and their toxins (adherent Listeria, Vibrio spp., shiga toxins), the use of antimicrobials to inhibit foodborne pathogens and spoilage microorganisms (bacteriocins, thermal destruction, intervention chemicals), and verification of food safety processes using inoculated validation studies.

Professional biography:

  • Assistant Professor, Department of Biological and Environmental Sciences, University of West Alabama (2017-present)
  • Post-doc Research Associate, Department of Animal Sciences, Oklahoma State University (2016-2017)
  • Ph.D. Graduate Research Associate, Department of Animal Science, Oklahoma State University (2009-2016)
  • Ph.D. Graduate Teaching Associate, Department of Animal Science, Oklahoma State University (2013-2017)

Selected presentations at professional meetings:

  • Efficacy of Homemade Electrolyzed Water Sanitizer for Inactivation of Foodborne Pathogens. Asma Elnaham, Hung King Tiong. International Association for Food Protection (IAFP), August 2, 2022. (Finalist work)

  • Characterization, Toxicity, and Antimicrobial Properties of Homemade Electrolyzed Water for Safe and Effective Disinfectant Applications. Asma Elnaham and Hung King Tiong. UWA University Research Symposium (URS), March 8, 2022. (2nd place work)

  • Isolation and Characterization of the Foodborne Pathogen Vibrio parahaemolyticus Variants Protected from Laboratory and Cooking Protocols from Retail Seafood Sources. Elizabeth Scruggs, Guadalupe Meza, Ahkayla R. Walker, and Hung King Tiong. International Association for Food Protection (IAFP), July 21, 2021.

  • Recovery of the Emerging Infectious Agent Vagococcus lutrae from Seafoods. Elizabeth Scruggs and Hung King Tiong. UWA University Research Symposium (URS), March 9, 2021. (Dean's award work)

  • Isolation, Characterization, and Identification of Potential Antimicrobial and Dental Care Lactic Acid Bacteria from Farm Animals, Produce, and Dental Sources. Mark Joseph Johnson, Erica Johnson, and Hung King Tiong. UWA University Research Symposium (URS), March 10, 2020.

  • Isolation and Characterization of Microorganisms from Food Loss/Waste Sources. Tori Fletcher, Ziyang Lin, and Hung King Tiong. UWA University Research Symposium (URS), March 10, 2020.

  • Evaluation of Seafood from Retail Sources for the Potential Presence of a Novel Group of Vibrio parahaemolyticus, the root of persistent vibriosis outbreak. Xiaoning Wei, Kayla Walker, Hung King Tiong. UWA University Research Symposium (URS), March 10, 2020. 
  • Isolation and Characterization of Vibrio parahaemolyticus Protected from Laboratory Validation from Natural Seafood Sources. International Association for Food Protection (IAFP), July 22, 2019. (Finalist work)
  • Isolation of Antimicrobial- and Lactase-Producing Lactic Acid Bacteria from Farm Animals and Produce. IAFP, July 23, 2019. (Finalist work)
  • Postharvest Interventions to Improve Quality and Microbial Safety of Microgreens. Institute of Food Technologists. Institute of Food Technologists (IFT), June 5, 2019.
  • Culture-based Isolation and Identification of Soil Microorganisms for Potential Soil Regenerating and Agricultural Benefits. UWA University Research Symposium (URS), March 5, 2019.
  • Regenerating Agricultural Soil of the Black Belt Region Using Bio-fertilizer. URS, March 6, 2018.
  • Identification of Putative Surface Proteins Involved in Adherence of L. monocytogenes on Abiotic Surfaces. IAFP, July 19, 2017.
  • Analysis of Surface Proteomics involved in Adherence of L. monocytogenes to Abiotic Surfaces. OSU Food & Agricultural Products Center/Institute of Food Technologists-Oklahoma (FAPC/IFT) Research Symposium, Feb 2017. (1st place work)
  • DNA Array of Select Genes of Two Surface-Adherent Phenotypes of L. monocytogenes Using PCR for Identification of Surface Adhesins. OSU FAPC/IFT-Oklahoma Research Symposium, Feb 2016.
  • Orbitrap Mass Spectrometry and qRT-PCR Analysis of Gene Expression to Identify the Molecular Basis of Bacterial Adherence in L. monocytogenes. OSU FAPC/IFT-Oklahoma Research Symposium, Feb 2013.
  • Genetics of Hydrogen Peroxide Production in S. pneumonia. American Society for Microbiology (ASM of Missouri branch), 2008.

Professional memberships:

  • The American Society for Microbiology (ASM)
  • The International Association for Food Protection (IAFP)
  • The Institute of Food Technologists (IFT)

Recent grants:

  • USDA Specialty Crop BLOCK Grant (2022-2023)
  • UWA Faculty Seed Grants (2021-2022; 2019-2021; 2017-2018)
  • UWA Student Seed Grants (2019-2021; 2017-2018)
  • USDA Specialty Crop BLOCK Grant (2018-2019)
  • UWA Internal Research Funds (2017-present)

Education

  • Ph.D. - Oklahoma State University, Stillwater, OK Food Science (Microbiology) 2016
  • M.S. - Oklahoma State University, Stillwater, OK Microbiology/Cell & Molecular Biology
  • B.S. - Oklahoma State University, Stillwater, OK Plant & Soil Sciences (Biotechnology)
  • Recent Publications

  • Guadalupe Meza, Hussain Majrshi, Kumar Saurabh Singh, Ratnakar Deole, and Hung King Tiong Draft Genome Sequences of the Vibrio parahaemolyticus Strains VHT1 and VHT2, Pasteurization-Resistant Isolates from Environmental Seafood. MRA. https://doi.org/10.1128/mra.00793-22. 2022-September
  • Asma Elnaham and Hung King Tiong Efficacy of Homemade Electrolyzed Water Sanitizer for Inactivation of Foodborne Pathogens. https://iafp.confex.com/iafp/2022/onlineprogram.cgi/Paper/28768 2022-August
  • Guadalupe Meza, Hussain Majrshi, Hung King Tiong. Recovery of Pasteurization-Resistant Vibrio parahaemolyticus from Seafoods Using a Modified, Two-Step Enrichment. Foods. https://doi.org/10.3390/foods11050764 2022-March
  • Scruggs, E. and H.K. Tiong Isolation and characterization of the foodborne pathogen Vibrio parahaemolyticus variants protected from laboratory and cooking protocols from retail seafood sources. https://iafp.confex.com/iafp/2021/onlineprogram.cgi/Paper/25895 2021-July
  • Truitt, Chelsea, G. Meza, H.K. Tiong, P. Hoyt, and R. Deole Bacterial genome of the novel Lactococcus lactis strain PrHT3. Biosample # SAMN19301478. NCBI SRA accession # SRS9038781 2021-May
  • Meza, G., H. Majrshi, K.S. Singh, R. Deole, and H.K. Tiong Bacterial genome of the novel Vibrio parahaemolyticus strain VHT1. NCBI SRA accession # SRR12395870 2020
  • Meza, G., H. Majrshi, K.S. Singh, R. Deole, and H.K. Tiong Bacterial genome of the novel Vibrio parahaemolyticus strain VHT2. NCBI SRA accession # SRR12395869 2020
  • Johnson, E., G. Meza, H.K. Tiong. Isolation of Antimicrobial- and Lactase-producing Lactic Acid Bacteria from Farm Animals and Produce. https://iafp.confex.com/iafp/2019/meetingapp.cgi/Paper/21740 2019
  • Walker, K., G. Meza, H.K. Tiong. Isolation and Characterization of Vibrio parahaemolyticus Protected from Laboratory Validation from Natural Seafood Sources. https://iafp.confex.com/iafp/2019/meetingapp.cgi/Paper/21462 2019
  • Tiong, H.K. and P.M. Muriana Identification of Putative Surface Proteins Involved in Adherence of Listeria monocytogenes on Abiotic Surfaces. https://iafp.confex.com/iafp/2017/webprogram/Paper15617.html 2017
  • Tiong, H.K. and P.M. Muriana. RT-qPCR Analysis of 15 Genes Encoding Putative Surface Proteins Involved in Adherence of Listeria monocytogenes. Pathogens 5(4):60. doi:10.3390/pathogens5040060 2016
  • Tiong, H.K., S. Hartson, and P.M. Muriana. Comparison of surface proteomes of adherence variants of Listeria monocytogenes using LC-MS/MS for identification of surface adhesins. Pathogens. 2016 Jun; 5(2):40. doi:10.3390/pathogens5020040 2016
  • Tiong, H.K., S. Hartson, and P.M. Muriana. Comparison of five methods for direct extraction of surface proteins from Listeria monocytogenes for proteomic analysis by Orbitrap mass spectrometry. J. Microbiol. Methods 110: 54-60. doi:10.1016/j.mimet.2015.01.004 2015